… has been an issue of interest in protein biotechnology. We thoroughly studied the hydrolysis
assisted by Ni(II) ions in R N –(Ser/Thr)–Xaa–His–Zaa–R C peptide sequences. First, we …

… sequence-specific cleavage of the peptide bond is a crucial procedure in protein engineering
… among metal ions and their complexes included redox-active metal ions. They can cleave …

… Ni(II)-assisted peptide bond hydrolysis of the tested peptides. … peptides in the 4N hydrolytically
active complex (Table 2) and … geometry optimization using a Dreiding-like forcefield [43]. …

… ion-catalyzed peptide bond cleavage … sequence-specific metal ion-catalyzed cleavage
show great promise, but further work is needed to fully delineate not only the optimal sequences …

… with the Ni(II)-dependent peptide bond hydrolysis, which … the hydrolysis reaction we used
the iterative optimization algorithm … Low-spin 4N complexes are hydrolytically active species. …

… Sequence-specific Ni(II)-dependent peptide bond hydrolysis for protein engineering.
Combinatorial library determination of optimal sequences …

… proteins to be hydrolyzed site-specifically by Ni 2+ ions; motif … optimized amino acid
sequence has been developed for Ni (… )-dependent peptide bond hydrolysis (39), a His 6 tag (…

… (II) ion which is also commonly found at active sites acts as a catalyst in the hydrolysis of
the peptide bond … The sequence-specific peptide bond cleavage was also studied in R N -(Ser/…

… Advanced protein engineering of enzymes derived from extremophiles is also … the hydrolysis
of peptide bonds. [ 26 ] This specific Pd(II)–CD complex was shown to be sequence specific, …

… may include the peptide bond hydrolysis in X 1 -S/Tc/pHcX 2 motifs by released Ni 2+ ions.
… 150 mM NaCl buffer with or without nickel ions [1 mM Ni(NO 3 ) 2 ] under optimal (pH 8.2, 50C…